1QOA
FERREDOXIN MUTATION C49S
Experimental procedure
Temperature [K] | 277 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.730, 38.230, 148.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.700 |
R-factor | 0.182 * |
Rwork | 0.182 |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | WILD TYPE |
RMSD bond length | 0.014 |
RMSD bond angle | 2.250 * |
Data reduction software | XDS |
Data scaling software | XSCALIBRE |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.780 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.029 | 0.105 |
Total number of observations | 58282 * | |
Number of reflections | 21258 * | 2175 * |
<I/σ(I)> | 27.8 | 4.2 |
Completeness [%] | 85.0 * | 66 |
Redundancy | 2.8 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.5 | 4 * | pH 5.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2.6 (M) | |
2 | 1 | reservoir | potassium succinate | 50 (mM) | |
3 | 1 | reservoir | MPD | 1 (%) |