1QNW

lectin II from Ulex europaeus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	287
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	104.910, 104.910, 175.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.350
R-factor	0.178
Rwork	0.178
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PHA-L AND MODEL FROM SWISS-MODEL SERVER
RMSD bond length	0.007
RMSD bond angle	27.132 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.350	2.350
Rmerge	0.134	0.542
Total number of observations	153615 *
Number of reflections	45367	4365 *
<I/σ(I)>	9.99	2.76
Completeness [%]	97.0	94.6
Redundancy	3.39	1.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		Dao-Thi M.H., (1998) Acta Crystallog. sect., D54, 844. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.0-5.0 (mg/ml)
2	1	reservoir	cacodylate	100 (mM)	or MES
3	1	reservoir	PEG6000	8-16 (%(w/v))