1QNN

Cambialistic superoxide dismutase from Porphyromonas gingivalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-08-29
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 21 21 21
Unit cell lengths	75.470, 102.660, 99.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.179
Rwork	0.179
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1isa
RMSD bond length	0.006
RMSD bond angle	23.900 *
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.400	1.860
High resolution limit [Å]	1.790	1.790
Rmerge	0.057	0.262
Number of reflections	64134
<I/σ(I)>	11.6	2
Completeness [%]	88.2	68.5
Redundancy	5.64

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8 *	293	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6-9 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	potassium phosphate	100 (mM)
4	1	reservoir	PEG4000	26-31 (%)