1QNL

AMIDE RECEPTOR/NEGATIVE REGULATOR OF THE AMIDASE OPERON OF PSEUDOMONAS AERUGINOSA (AMIC) COMPLEXED WITH BUTYRAMIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-12-15
Detector	MARRESEARCH
Spacegroup name	P 42 21 2
Unit cell lengths	104.150, 104.150, 65.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.24 *
Rwork	0.269
R-free	0.31400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pea
RMSD bond length	0.007
RMSD bond angle	0.030
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	2.840
High resolution limit [Å]	2.700	2.700
Rmerge	0.098	0.185
Number of reflections	10389
<I/σ(I)>	4.5	3.8
Completeness [%]	97.8	97.2
Redundancy	2.7	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	7		Chayen, N.E., (1992) J. Crystal Growth, 122, 176. *