1QNL
AMIDE RECEPTOR/NEGATIVE REGULATOR OF THE AMIDASE OPERON OF PSEUDOMONAS AERUGINOSA (AMIC) COMPLEXED WITH BUTYRAMIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 104.150, 104.150, 65.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.24 * |
| Rwork | 0.269 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pea |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.840 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.098 | 0.185 |
| Number of reflections | 10389 | |
| <I/σ(I)> | 4.5 | 3.8 |
| Completeness [%] | 97.8 | 97.2 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 7 | Chayen, N.E., (1992) J. Crystal Growth, 122, 176. * |
