1QN2

cytochrome cH from Methylobacterium extorquens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF NONIUS
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-04-15
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	33.760, 57.570, 50.950
Unit cell angles	67.81, 89.33, 74.40

Refinement procedure

Resolution	30.000 - 2.010
Rwork	0.161
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c2c
RMSD bond length	0.007
RMSD bond angle	0.018
Data reduction software	MOSFLM
Data scaling software	Agrovata
Phasing software	CCP4
Refinement software	CCP4

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.010
Rmerge	0.061	0.134
Number of reflections	16999
<I/σ(I)>	16.3	6.3
Completeness [%]	94.4	71.5
Redundancy	2.25	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7		HANGING DROP VAPOUR DIFFUSION, 10 MG/ML PROTEIN, 18% PEG 1500, 50 MM TRIS-HCL, PH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG1500	18 (%)
3	1	reservoir	Tris-HCl	50 (mM)