1QMZ

PHOSPHORYLATED CDK2-CYCLYIN A-SUBSTRATE PEPTIDE COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-12-22
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	152.600, 163.700, 73.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
Rwork	0.220
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CDK2-CYCLIN A-RECRUITMENT PEPTIDE STRUCTURE SUBMITTED ALONG WITH THIS STRUCTURE
RMSD bond length	0.019
RMSD bond angle	0.048
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.120	0.510
Number of reflections	51615
<I/σ(I)>	8.1	2.5
Completeness [%]	95.4	95.4
Redundancy	2.6	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7	4 *	pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	drop	peptide	1 (mM)
3	1	drop	AMPPNP	1 (mM)