1QMP

Phosphorylated aspartate in the crystal structure of the sporulation response regulator, Spo0A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1997-10-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	69.197, 72.859, 114.297
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.000
R-factor	0.2
Rwork	0.195
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	N-SPO0A IN DIFFERENT CRYSTAL FORM (YET TO BE DEPOSITED)
RMSD bond length	0.011
RMSD bond angle	0.030
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.085 *	0.293 *
Total number of observations	173534 *
Number of reflections	39774
<I/σ(I)>	17.5	5.2
Completeness [%]	99.9	99.9
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		225 MM CACL2, 100 MM MOPS, PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MOPS
2	1	reservoir		225 (mM)