1QMO

Structure of FRIL, a legume lectin that delays hematopoietic progenitor maturation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	287
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 65 2 2
Unit cell lengths	151.360, 151.360, 309.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.500
R-factor	0.232
Rwork	0.232
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MODEL
RMSD bond length	0.008
RMSD bond angle	26.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (0.4)
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.620
High resolution limit [Å]	3.500	3.500
Rmerge	0.159 *
Total number of observations	257389 *
Number of reflections	26919 *
<I/σ(I)>	11.3	4.8
Completeness [%]	99.4
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		METHOD 'HANING DROP BOTTOM', 20 % PEG 8000, 0.1 M NACACODYLATE, PH 6.5, 0.2 M (NH4)2SO4, WITH A 5 MICROLITER PROTEIN SOLUTION DROP, (4.3 MG/ML)+ 5 MICROLITER BOTTOM SOLUTION + 1 MICROLITER TRIMANNOSIDE SOLUTION (90 MM)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.3 (mg/ml)
2	1	drop	Man(alpha1-3)[Man(alpha1-6)]Man(alpha1-O-Me)	90 (mM)
3	1	drop	bottom solution
4	1	reservoir	PEG8000	11 (%)	bottom solution
5	1	reservoir	Na cacodylate	0.1 (M)	bottom solution
6	1	reservoir	ammonium sulfate	0.2 (M)	bottom solution