1QMH

Crystal structure of RNA 3'-terminal phosphate cyclase, an ubiquitous enzyme with unusual topology

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	1998-01-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	125.800, 133.500, 51.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.207
Rwork	0.204
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qmi CHAIN A
RMSD bond length	0.022
RMSD bond angle	0.046
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.043 *	0.268 *
Total number of observations	159690 *
Number of reflections	50057
<I/σ(I)>	11.3	3.6
Completeness [%]	97.2	83.4
Redundancy	3.2	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5	20 *	THE PROTEIN WAS CRYSTALLIZED FROM 13-15% MPEG 2000, 200 MM NA-CITRATE PH 4.0, 200 MM TRIS/HCL PH 8.0, 2 MM DTT. PROTEIN CONCENTRATION WAS CA. 15 MG/ML.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	mPEG2000	13-15 (%)
3	1	reservoir	Na citrate	200 (mM)
4	1	reservoir	Tris-HCl	200 (mM)
5	1	reservoir	dithiothreitol	2 (mM)