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1QMH

Crystal structure of RNA 3'-terminal phosphate cyclase, an ubiquitous enzyme with unusual topology

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X9B
Synchrotron siteNSLS
BeamlineX9B
Temperature [K]105
Detector technologyIMAGE PLATE
Collection date1998-01-15
DetectorMARRESEARCH
Spacegroup nameP 21 21 2
Unit cell lengths125.800, 133.500, 51.000
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution10.000 - 2.100
R-factor0.207
Rwork0.204
R-free0.27600
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1qmi CHAIN A
RMSD bond length0.022
RMSD bond angle0.046
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareREFMAC
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.170
High resolution limit [Å]2.1002.100
Rmerge0.043

*

0.268

*

Total number of observations159690

*

Number of reflections50057
<I/σ(I)>11.33.6
Completeness [%]97.283.4
Redundancy3.22.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

5.520

*

THE PROTEIN WAS CRYSTALLIZED FROM 13-15% MPEG 2000, 200 MM NA-CITRATE PH 4.0, 200 MM TRIS/HCL PH 8.0, 2 MM DTT. PROTEIN CONCENTRATION WAS CA. 15 MG/ML.
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein15 (mg/ml)
21reservoirmPEG200013-15 (%)
31reservoirNa citrate200 (mM)
41reservoirTris-HCl200 (mM)
51reservoirdithiothreitol2 (mM)

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PDB entries from 2024-05-15

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