1QMA

Nuclear Transport Factor 2 (NTF2) W7A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	64.652, 75.407, 64.543
Unit cell angles	90.00, 115.00, 90.00

Refinement procedure

Resolution	6.000 - 2.500
Rwork	0.208
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oun
RMSD bond length	0.012
RMSD bond angle	0.033
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.700	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.082	0.194
Total number of observations	65817 *
Number of reflections	18928
<I/σ(I)>	6.1	3.5
Completeness [%]	97.4	91.8
Redundancy	2.5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	18 *	pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.5 (mg/ml)
2	1	reservoir	ammonium sulfate	1.32 (M)
3	1	reservoir	isopropanol	3 (%(v/v))
4	1	reservoir	Tris-HCl	100 (mM)