1QMA
Nuclear Transport Factor 2 (NTF2) W7A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.652, 75.407, 64.543 |
| Unit cell angles | 90.00, 115.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| Rwork | 0.208 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oun |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.033 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.700 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.082 | 0.194 |
| Total number of observations | 65817 * | |
| Number of reflections | 18928 | |
| <I/σ(I)> | 6.1 | 3.5 |
| Completeness [%] | 97.4 | 91.8 |
| Redundancy | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 18 * | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.5 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.32 (M) | |
| 3 | 1 | reservoir | isopropanol | 3 (%(v/v)) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) |
