1QM8
Structure of Bacteriorhodopsin at 100 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-18 |
| Detector | FUJI FILM |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 102.100, 102.100, 112.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bm1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 20.630 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.057 * | 0.387 * |
| Number of reflections | 12500 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 9.9 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.2 | Kouyama, T., (1994) J. Mol. Biol., 236, 990. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4-7 (mg/ml) | |
| 2 | 1 | drop | octylthioglucoside | 20 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 0.7-1.2 (M) | |
| 4 | 1 | drop | sodium citrate | 30-50 (mM) | |
| 5 | 1 | drop | 150 (mM) | ||
| 6 | 1 | drop | 0.07 (%) |
