1QM4
Methionine Adenosyltransferase Complexed with a L-Methionine Analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 115.200, 115.200, 159.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.660 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mxa |
| RMSD bond length | 0.017 |
| RMSD bond angle | 26.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 2.800 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.082 | 0.370 * |
| Number of reflections | 28742 | |
| <I/σ(I)> | 6.7 | 2.1 |
| Completeness [%] | 94.5 | 85.8 |
| Redundancy | 7.5 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | drop consists of equal amounts of protein and precipitant solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | L-cisAMB | 10 (mM) | |
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | drop | 50 (mM) | ||
| 6 | 1 | drop | 10 (mM) | ||
| 7 | 1 | drop | EDTA | 1 (mM) | |
| 8 | 1 | reservoir | PEG10000 | 16 (%(w/v)) | precipitant |
| 9 | 1 | reservoir | HEPES | 100 (mM) | precipitant |
