1QLS

S100C (S100A11),OR CALGIZZARIN, IN COMPLEX WITH ANNEXIN I N-TERMINUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE D41A
Synchrotron site	LURE
Beamline	D41A
Temperature [K]	280
Detector technology	IMAGE PLATE
Collection date	1998-07-02
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	77.510, 77.510, 111.610
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
Rwork	0.214
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bt6
RMSD bond length	0.017
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.300
Rmerge	0.080
Total number of observations	61772 *
Number of reflections	9310
<I/σ(I)>	20.1
Completeness [%]	99.7
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		drop consists of 1:1 mixture of well and protein solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG4000	20 (%)
3	1	reservoir	2-propanol	10 (%)
4	1	reservoir	HEPES	100 (mM)