1QL9
FACTOR XA SPECIFIC INHIBITOR IN COMPLEX WITH RAT TRYPSIN MUTANT X99RT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 124.140, 124.140, 124.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| Structure solution method | OTHER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.456 |
| Total number of observations | 57863 * | |
| Number of reflections | 16310 | |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | a single crystal was soaked * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 13.5 (mg/ml) | |
| 2 | 1 | 1 | benzamidine | 100 (mM) | |
| 3 | 1 | 1 | Tris-HCl | 25 (mM) | |
| 4 | 1 | 1 | 10 (mM) | ||
| 5 | 1 | 1 | 40 (%) |
