1QL0

Sm Endonuclease from Seratia marcenscens at atomic resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	120
Spacegroup name	P 21 21 2
Unit cell lengths	106.390, 73.670, 68.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.100
R-factor	0.129 *
R-free	0.15600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	4.500 *
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.100
Rmerge	0.041 *	0.252 *
Number of reflections	198103 *
Completeness [%]	91.5
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	6.5		Bannikova, G.E., (1991) Biochem. Int., 23, 813. *