1QKR
Crystal structure of the vinculin tail and a pathway for activation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.600, 87.770, 50.630 |
| Unit cell angles | 90.00, 91.27, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.24800 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 17.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.5) |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.064 * | 0.270 * |
| Number of reflections | 30415 | |
| <I/σ(I)> | 16.6 | 3 |
| Completeness [%] | 98.6 * | 88.3 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 * | 22 * | 25% (W/V) PEG 2000, 0.2 M (NH4)2SO4, 0.1 M CH3COO PH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | Tris | 20 (mM) | |
| 4 | 1 | drop | dithiothreitol | 4 (mM) | |
| 5 | 1 | reservoir | PEG2000 | 25 (%(w/v)) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 7 | 1 | reservoir | sodium acetate | 0.1 (M) |
