1QKQ

CHARCOT-LEYDEN CRYSTAL PROTEIN - MANNOSE COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-03-15
Detector	MARRESEARCH
Spacegroup name	P 65 2 2
Unit cell lengths	49.600, 49.600, 263.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 1.800
R-factor	0.236
Rwork	0.236
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lcl
RMSD bond length	0.006
RMSD bond angle	29.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.074 *
Total number of observations	138775 *
Number of reflections	18942
<I/σ(I)>	7.8
Completeness [%]	99.3	98.7
Redundancy	3.3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	10.5 *	16 *	02M TRIS.HCL PH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	0.1 (M)
3	1	reservoir	Tris-acetate	0.2 (M)