1QJZ
Three Dimensional Structure of Physalis Mottle Virus : Implications for the Viral Assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 295 |
| Detector technology | OSCILLATION CAMERA |
| Spacegroup name | R 3 |
| Unit cell lengths | 294.650, 294.650, 294.650 |
| Unit cell angles | 59.91, 59.91, 59.91 |
Refinement procedure
| Resolution | 10.000 - 3.800 |
| R-factor | 0.326 |
| Rwork | 0.326 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1auy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 27.000 * |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 60.000 |
| High resolution limit [Å] | 3.800 |
| Number of reflections | 137259 |
| Completeness [%] | 40.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | pH 5.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | virus | 30-40 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 2 (mM) | |
| 3 | 1 | drop | sodium citrate | 0.1 (M) | |
| 4 | 1 | reservoir | PEG6000 | 2.5 (%) | inner well |
| 5 | 1 | reservoir | PEG6000 | 10 (%) | outer well |
