1QI4
MUTANT (E219G) MALTOTETRAOSE-FORMING EXO-AMYLASE IN COMPLEX WITH MALTOTETRAOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.520, 171.100, 46.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.18 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1jdd |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.035 |
| Phasing software | PHASER |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.077 |
| Total number of observations | 97510 * |
| Number of reflections | 32330 |
| Completeness [%] | 87.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 0.7-0.9 (M) | |
| 4 | 1 | drop | maltopentaose | 10 (mg/ml) |
