1QI3

MUTANT (D193N) MALTOTETRAOSE-FORMING EXO-AMYLASE IN COMPLEX WITH MALTOTETRAOSE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	65.770, 170.800, 46.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.186
Structure solution method	OTHER
Starting model (for MR)	1jdd
RMSD bond length	0.014
RMSD bond angle	0.032
Phasing software	PHASER
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	62.000
High resolution limit [Å]	2.000
Rmerge	0.062
Total number of observations	128837 *
Number of reflections	33736
Completeness [%]	91.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	20 *	pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop	ammonium sulfate	0.7-0.9 (M)
4	1	reservoir	maltopentaose	10 (mg/ml)