1QHV

HUMAN ADENOVIRUS SEROTYPE 2 FIBRE HEAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-04-01
Detector	MARRESEARCH
Spacegroup name	P 3 2 1
Unit cell lengths	95.350, 95.350, 48.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.970 - 1.510
R-factor	0.109 *
Rwork	0.109
R-free	0.14300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1knb
RMSD bond length	0.016
RMSD bond angle	0.014
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.970	1.590
High resolution limit [Å]	1.510	1.510
Rmerge	0.073 *	0.172 *
Total number of observations	386827 *
Number of reflections	36820	3170 *
<I/σ(I)>	7.3	3.6
Completeness [%]	91.8	55.3
Redundancy	4.7	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4	20 *	100 MM SODIUM ACETATE 25 % (V/V) GLYCEROL 1-1.5 M AMMONIUM SULPHATE PH 4.0 WITH ACETIC ACID

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.0-1.5 (M)
2	1	reservoir	sodium sulfate	0.1 (M)
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir	glycerol	25 (%(v/v))
5	1	drop	protain	20 (mg/ml)
6	1	drop	dithiothreitol	1 (mM)