1QHV
HUMAN ADENOVIRUS SEROTYPE 2 FIBRE HEAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 95.350, 95.350, 48.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.970 - 1.510 |
| R-factor | 0.109 * |
| Rwork | 0.109 |
| R-free | 0.14300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1knb |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.014 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.970 | 1.590 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.073 * | 0.172 * |
| Total number of observations | 386827 * | |
| Number of reflections | 36820 | 3170 * |
| <I/σ(I)> | 7.3 | 3.6 |
| Completeness [%] | 91.8 | 55.3 |
| Redundancy | 4.7 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4 | 20 * | 100 MM SODIUM ACETATE 25 % (V/V) GLYCEROL 1-1.5 M AMMONIUM SULPHATE PH 4.0 WITH ACETIC ACID |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.0-1.5 (M) | |
| 2 | 1 | reservoir | sodium sulfate | 0.1 (M) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 4 | 1 | reservoir | glycerol | 25 (%(v/v)) | |
| 5 | 1 | drop | protain | 20 (mg/ml) | |
| 6 | 1 | drop | dithiothreitol | 1 (mM) |
