1QHU
MAMMALIAN BLOOD SERUM HAEMOPEXIN DEGLYCOSYLATED AND IN COMPLEX WITH ITS LIGAND HAEM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.680, 61.950, 83.290 |
| Unit cell angles | 90.00, 93.21, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.201 |
| Rwork | 0.207 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1HXN AND 1FBL |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.324 |
| Number of reflections | 19233 | |
| <I/σ(I)> | 6 | 2.1 |
| Completeness [%] | 94.7 | 77 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * | HANGING DROP, 4 DEGREES CENTIGRADES, RESEVOIR SOLUTION: 19-22% PEG 4000, 0.05 M TRIS HCL PH 7.5, 0.05-0.5 M EDTA, 0.2 NACL PROTEIN COMPLEX SOLUTION: 40 MG/ ML IN 0.05 M TRIS PH 7.0 AND 0.2 M NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 19-22 (%(w/v)) | |
| 2 | 1 | reservoir | Tris-HCl | 0.05 (M) | |
| 3 | 1 | reservoir | EDTA | 0.05-0.5 (M) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | drop | protain | 40 (mg/ml) | |
| 6 | 1 | drop | Tris-HCl | 0.05 (M) | |
| 7 | 1 | drop | 0.2 (M) |
