1QHN
CHLORAMPHENICOL PHOSPHOTRANSFERASE FROM STREPTOMYCES VENEZUELAE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.9785, 0.9783, 0.88 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 200.000, 200.000, 200.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.000 - 2.700 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.780 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.128 | 0.368 |
| Total number of observations | 255940 * | |
| Number of reflections | 18936 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 13.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 0.4 (M) | |
| 4 | 1 | drop | acetonitrile | 4 (%) | |
| 5 | 1 | drop | Cm | 5 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 7 | 1 | reservoir | ammonium sulfate | 1.14 (M) | |
| 8 | 1 | drop | magnesium chloride | 10 (mM) |
