1QH4
CRYSTAL STRUCTURE OF CHICKEN BRAIN-TYPE CREATINE KINASE AT 1.41 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-22 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.430, 175.990, 95.400 |
| Unit cell angles | 90.00, 95.85, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.410 |
| R-factor | 0.134 * |
| R-free | 0.18810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1crk MITOCHONDRIAL CREATINE KINASE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.029 |
| Data reduction software | XDS (V. 99) |
| Data scaling software | XDS (V. 99) |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.500 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.042 | 0.215 |
| Number of reflections | 301191 | 49959 * |
| <I/σ(I)> | 17.2 | 5 |
| Completeness [%] | 99.1 | 97.1 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | 20 * | 0.1M MES, 0.4M CA(OAC)2, 15% PE 2MM DTT, PH 6.5, 8 MG/ML PROTEIN |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | MES | 25 (mM) | |
| 3 | 1 | drop | dithiothreitol | 3 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | reservoir | 0.4 (M) | ||
| 6 | 1 | reservoir | PEG8000 | 15 (%) | |
| 7 | 1 | reservoir | MES | 0.1 (M) |
