1QGZ
FERREDOXIN:NADP+ REDUCTASE MUTANT WITH LEU 78 REPLACED BY ASP (L78D)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Collection date | 1998-02 |
Detector | MARRESEARCH |
Spacegroup name | P 65 |
Unit cell lengths | 88.030, 88.030, 97.260 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 2.300 |
R-factor | 0.178 * |
Rwork | 0.177 |
R-free | 0.22700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1que |
RMSD bond length | 0.007 |
RMSD bond angle | 26.190 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.600 * | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.081 | 0.224 |
Number of reflections | 18686 | |
<I/σ(I)> | 8.2 | 3.4 |
Completeness [%] | 99.4 | 96 * |
Redundancy | 7.7 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 20 * | pH 5.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.75 (mM) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
3 | 1 | drop | beta-octylglucoside | 5 (%(w/v)) | unbuffered |
4 | 1 | reservoir | PEG6000 | 18-20 (%(w/v)) | |
5 | 1 | reservoir | ammonium sulfate | 20 (mM) | |
6 | 1 | reservoir | MES- NaOH | 0.1 (M) | pH5.0 |