1QGY
Ferredoxin:NADP+ reductase mutant with Lys 75 replaced by Glu (K75E)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM02 |
| Synchrotron site | ESRF |
| Beamline | BM02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-02-15 |
| Spacegroup name | P 65 |
| Unit cell lengths | 87.000, 87.000, 96.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.000 - 1.700 |
| Rwork | 0.200 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1que |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.888 |
| Data reduction software | AUTOMAR |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.300 | |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.051 | 0.118 |
| Number of reflections | 37317 | |
| <I/σ(I)> | 48.56 | 17 |
| Completeness [%] | 86.6 | 50.3 |
| Redundancy | 3.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | pH 5.0 |
