1QGW
CRYSTAL STRUCTURE OF PHYCOERYTHRIN 545 FROM THE MARINE CRYPTOPHYTE RHODOMONAS CS24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-01 |
| Detector | MACSCIENCE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.030, 82.630, 89.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.630 |
| R-factor | 0.149 * |
| Rwork | 0.149 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BETA CHAIN FROM PDB ENTRY 1LIA |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.019 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.670 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.039 | 0.104 |
| Total number of observations | 264395 * | |
| Number of reflections | 56582 * | |
| <I/σ(I)> | 21.3 | 12 |
| Completeness [%] | 95.8 | 79.4 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 * | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%) | |
| 2 | 1 | reservoir | 100 (mM) | ||
| 3 | 1 | reservoir | 50 (mM) | ||
| 4 | 1 | reservoir | Tris | 50 (mM) | |
| 5 | 1 | drop | protain | 20 (mg/ml) | |
| 6 | 1 | drop | MES | 50 (mM) |
