1QGV
HUMAN SPLICEOSOMAL PROTEIN U5-15KD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.225, 65.092, 36.275 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| Rwork | 0.216 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 26.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.029 * | 0.265 * |
| Total number of observations | 130566 * | |
| Number of reflections | 28054 | |
| Completeness [%] | 95.2 | 86.1 |
| Redundancy | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 294 | 1.0 M AMMONIUM SULPHATE 0.2 M HEPES/NAOH, PH 7.6 AT 294 K, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 100 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 120 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 7 | 1 | reservoir | sodium azide | 0.02 (%(w/v)) | |
| 8 | 1 | reservoir | ammonium sulfate | 1 (M) | |
| 9 | 1 | reservoir | HEPES/NaOH | 200 (mM) |
