1QGH
THE X-RAY STRUCTURE OF THE UNUSUAL DODECAMERIC FERRITIN FROM LISTERIA INNOCUA, REVEALS A NOVEL INTERSUBUNIT IRON BINDING SITE.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Collection date | 1997-12-01 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.540, 137.500, 173.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.350 |
| Rwork | 0.219 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dps |
| RMSD bond length | 0.012 |
| RMSD bond angle | 14.698 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.350 |
| Rmerge | 0.077 * |
| Number of reflections | 80150 |
| Completeness [%] | 91.3 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.8 | 293 * | PEG 1000 AT 19-25 %, MES 0.1 M AT PH 5.0-6.2, pH 5.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | |
| 3 | 1 | reservoir | PEG1000 | 19-25 (%) |
