1QGA
PEA FNR Y308W MUTANT IN COMPLEX WITH NADP+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-02-01 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.400, 110.300, 80.600 |
| Unit cell angles | 90.00, 93.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Y308S:NADP+ |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.059 * | 0.314 * |
| Total number of observations | 224800 * | |
| Number of reflections | 57761 | |
| <I/σ(I)> | 9.8 | 2.6 |
| Completeness [%] | 97.5 | 96.2 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 25 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.5 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 5 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.1 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) |
