1QG8
NATIVE (MAGNESIUM-CONTAINING) SPSA FROM BACILLUS SUBTILIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-06-01 |
| Detector | ADSC |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.420, 141.997, 81.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| Rwork | 0.160 |
| R-free | 0.21000 |
| Structure solution method | MIR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.034 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.800 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.037 | 0.270 |
| Number of reflections | 43230 | |
| <I/σ(I)> | 22.5 | 3.1 |
| Completeness [%] | 97.0 | 88 * |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | Charnock, S.J., (1999) Acta Crystallogr., D55, 677. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | HEPES | 100 (mM) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | reservoir | PEG8000 | 15 (%(w/v)) |
