1QG5

HIGH RESOLUTION CRYSTAL STRUCTURE OF THE BOVINE BETA-LACTOGLOBULIN (ISOFORM A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE D03B-MX1
Synchrotron site	LNLS
Beamline	D03B-MX1
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	1997-09-15
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	55.470, 82.110, 66.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.500 - 2.000
Rwork	0.192
R-free	0.28300 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011 *
RMSD bond angle	2.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.900 *	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.066 *	0.460 *
Number of reflections	9631
<I/σ(I)>	12.7
Completeness [%]	90.9	91.2
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9		THE CRYSTAL WAS GROWN AT PH 7.9, HANGING DROP METHOD. DROP CONTAINED 2.5M (NH4) 2SO4, 180MM TRIZMA BUFFER, AND 20MG/ML PROTEIN REMARK 290, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	ammonium sulfate	2.5 (M)
3	1	reservoir	Tris-HCl	180 (mM)