1QFY
PEA FNR Y308S MUTANT IN COMPLEX WITH NADP+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-02-01 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.280, 110.650, 81.040 |
Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.800 |
R-factor | 0.195 * |
Rwork | 0.196 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | WILD-TYPE PEA FNR |
RMSD bond length | 0.015 |
RMSD bond angle | 1.790 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.064 * | 0.261 * |
Total number of observations | 277996 * | |
Number of reflections | 65999 | |
<I/σ(I)> | 9 | 3.1 |
Completeness [%] | 81.0 | 66.9 * |
Redundancy | 4.2 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | 25 * | pH 8.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.5 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 5 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 2.1 (M) | |
4 | 1 | reservoir | Tris-HCl | 0.1 (M) |