1QFV

HISTAMINE BINDING PROTEIN FROM FEMALE BROWN EAR RHIPICEPHALUS APPENDICULATUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Spacegroup name	P 21 21 21
Unit cell lengths	77.560, 74.400, 77.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.360
R-factor	0.193 *
Rwork	0.182
R-free	0.21300 *
Structure solution method	MIR
RMSD bond length	0.012 *
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.360
Rmerge	0.183 *
Number of reflections	108735
Completeness [%]	74.8
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	MES	0.1 (M)
3	1	reservoir	cobalt chloride hexahydrate	0.01 (M)
4	1	reservoir	ammonium sulfate	1.8 (M)