1QFC
STRUCTURE OF RAT PURPLE ACID PHOSPHATASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | I 41 |
| Unit cell lengths | 116.380, 116.380, 63.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.234 * |
| Rwork | 0.234 |
| R-free | 0.28100 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.107 * | |
| Number of reflections | 10486 | |
| <I/σ(I)> | 8 | 2 |
| Completeness [%] | 90.5 | 70.1 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.4 * | 18 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 16 (%(w/v)) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | |
| 3 | 1 | reservoir | potassium phosphate | 0.1 (M) | |
| 4 | 1 | drop | protein | 5 (mg/ml) | |
| 5 | 1 | drop | ammonium acetate | 10 (mM) |
