1QF8
TRUNCATED FORM OF CASEIN KINASE II BETA SUBUNIT (2-182) FROM HOMO SAPIENS
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.88, 0.978668, 0.978418 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 132.230, 132.230, 63.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.740 |
| R-factor | 0.1933 * |
| Rwork | 0.194 |
| R-free | 0.21900 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.043 | 0.326 |
| Total number of observations | 634684 * | |
| Number of reflections | 62119 * | |
| <I/σ(I)> | 34 | 7.05 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 10.2 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9.2 * | 281 | Chantalat, L., (1999) Acta Crystallogr., D55, 895. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | reservoir | mPEG5000 | 14-20 (%) | |
| 3 | 1 | reservoir | bicine | ||
| 4 | 1 | reservoir | 0.46 (M) | ||
| 5 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 6 | 1 | reservoir | dioxane | 3 (%) | |
| 7 | 1 | reservoir | 0.4 (M) |
