1QF6
STRUCTURE OF E. COLI THREONYL-TRNA SYNTHETASE COMPLEXED WITH ITS COGNATE TRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 162.670, 162.670, 128.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| Rwork | 0.195 |
| R-free | 0.23900 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 26.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.074 * | 0.337 * |
| Total number of observations | 176615 * | |
| Number of reflections | 43094 | |
| <I/σ(I)> | 16.8 | 5.9 |
| Completeness [%] | 98.5 | 98.5 |
| Redundancy | 4.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | AMMONIUM ACETATE, PEG 4000, PH 6.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | AMMONIUM ACETATE | ||
| 2 | 1 | 1 | PEG 4000 | ||
| 3 | 1 | 2 | AMMONIUM ACETATE | ||
| 4 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 3 | 1 | 1 | ammonium acetate | ||
| 4 | 1 | 1 | ATP | ||
| 5 | 1 | 2 | PEG4000 | precipitant |
