1QEW

HUMAN CLASS I HISTOCOMPATIBILITY ANTIGEN (HLA-A 0201) COMPLEX WITH A NONAMERIC PEPTIDE FROM MELANOMA-ASSOCIATED ANTIGEN 3 (RESIDUES 271-279)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-21
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	1999-03
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	58.990, 54.520, 76.380
Unit cell angles	90.00, 105.67, 90.00

Refinement procedure

Resolution	20.000 - 2.200
Rwork	0.204
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b0g
RMSD bond length	0.006
RMSD bond angle	1.290
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	22410
<I/σ(I)>	10.2	4.5
Completeness [%]	95.9	86.3
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		20 % POLYETHYLENGLYCOL 8000, SODIUM CITRATE, PH 5.6