1QDW

N-TERMINAL DOMAIN, VOLTAGE-GATED POTASSIUM CHANNEL KV1.2 RESIDUES 33-119

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1998-12-22
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	76.260, 78.740, 125.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.226 *
Rwork	0.223
R-free	0.26500 *
RMSD bond length	0.006 *
RMSD bond angle	1.130 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.076 *	0.283 *
Number of reflections	42293
<I/σ(I)>	10.9
Completeness [%]	94.5 *	83.7
Redundancy	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	18 *	9% PEG 1500, 5% N-PROPANOL, 120 MM GDHCL 50 MM MES PH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir	PEG1500	9 (%)
3	1	reservoir	n-propanol	5 (%)
4	1	reservoir	Gdm HCl	120 (mM)
5	1	reservoir	MES	50 (mM)
6	1	reservoir	water	0-15 (%)