1QDT
2.1 A RESOLUTION STRUCTURE OF ESCHERICHIA COLI LYTIC TRANSGLYCOYSLASE SLT35 IN COMPLEX WITH CALCIUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-21 |
| Detector | MAC Science DIP-2030 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.417, 67.788, 99.149 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.162 |
| Rwork | 0.159 |
| R-free | 0.20600 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.063 | 0.274 |
| Number of reflections | 21908 | |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 92.6 | 91 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 295 | PEG 20K, BICINE-NAOH, ISOPROPANOL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.0 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | isopropanol | 1 (%) | |
| 4 | 1 | drop | phenylmethylsulfonyl fluoride | 1 (mM) | |
| 5 | 1 | reservoir | Bicine-NaOH | 100 (mM) | |
| 6 | 1 | reservoir | PEG20K | 0-6 (%) |
