1QDO
MAN(APLHA1-3)MAN(ALPHA1-O)METHYL CONCANAVALIN A COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.410, 64.530, 126.960 |
| Unit cell angles | 90.00, 93.21, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.800 |
| R-factor | 0.164 * |
| Rwork | 0.164 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1enq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 27.393 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.168 | 0.603 |
| Total number of observations | 195361 * | |
| Number of reflections | 24411 | |
| <I/σ(I)> | 17.1 | 3.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | DROP: 5 MICROLITER 5 MG/ML CON A, 5 MM SUGAR + 5 MICROLITER 14% PEG 8000, 35 MM POTASSIUM PHOSPHATE; 500 MICROLITER BOTTOM SOLUTION, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ConA | 5 (mg/ml) | |
| 2 | 1 | drop | M3M | 5 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 14 (%) | |
| 4 | 1 | reservoir | potassium phosphate | 35 (mM) |
