1QDE
CRYSTAL STRUCTURE OF THE ATPASE DOMAIN OF TRANSLATION INITIATION FACTOR 4A FROM SACCHAROMYCES CEREVISIAE-THE PROTOTYPE OF THE DEAD BOX PROTEIN FAMILY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.880, 59.280, 92.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
R-factor | 0.213 * |
Rwork | 0.214 |
R-free | 0.26600 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.090 | 0.315 |
Number of reflections | 14988 | |
<I/σ(I)> | 14.5 | |
Completeness [%] | 99.6 | 99.1 |
Redundancy | 6.07 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.8 * | 20 * | PEG 8000, MGCL2, (NH4)2SO4, MES, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 5 (mM) | |
2 | 1 | drop | protein | 20 (mg/ml) | |
3 | 1 | reservoir | PEG8000 | 20 (%) | |
4 | 1 | reservoir | 0.01 (M) | ||
5 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
6 | 1 | reservoir | MES | 0.1 (M) |