1QDC
MAN(APLHA1-6)MAN(ALPHA1-O)METHYL CONCANAVALIN A COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.510, 117.030, 120.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.175 * |
| Rwork | 0.175 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1enq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 27.358 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.082 | 0.320 |
| Total number of observations | 498686 * | |
| Number of reflections | 71100 | |
| <I/σ(I)> | 17 | 6.3 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 291 | DROP: 5 MICROLITER 7 MG/ML CON A + 5 MICROLITER 7% JEFFAMINE M-600, 10 % PEG 8000, 50 MM NH4COOH, 5MM MGCL2, 25 MM PHOSPHATE, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ConA | 7 (mg/ml) | |
| 2 | 1 | drop | M6M | 2.75 (mM) | |
| 3 | 1 | reservoir | Jeffamine M-600 | 7 (%) | |
| 4 | 1 | reservoir | PEG8000 | 10 (%) | |
| 5 | 1 | reservoir | ammonium formate | 50 (mM) | |
| 6 | 1 | reservoir | 5 (mM) | ||
| 7 | 1 | reservoir | phosphate | 25 (mM) |
