1QD6

OUTER MEMBRANE PHOSPHOLIPASE A FROM ESCHERICHIA COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1998-09-13
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	80.067, 84.038, 95.245
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.300 - 2.100
R-factor	0.226
Rwork	0.226
R-free	0.28300
Starting model (for MR)	MONOMER OMPLA (1QD5) WITHOUT WATER/DETERGENT MOLECULES
RMSD bond length	0.007
RMSD bond angle	27.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.300	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.077	0.265
Number of reflections	28336
<I/σ(I)>	10.9
Completeness [%]	74.1	66.7
Redundancy	2.69

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5 *	293	protein solution is mixed in a 3:2 ratio with well solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	succinate	10 (mM)
3	1	drop	beta-OG	1.5 (%)
4	1	drop		1 (mM)
5	1	reservoir		1 (mM)
6	1	reservoir	bis-Tris	0.1 (M)