1QD5

OUTER MEMBRANE PHOSPHOLIPASE A FROM ESCHERICHIA COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1996-05-16
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	78.550, 78.550, 101.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.600 - 2.170
R-factor	0.221
Rwork	0.221
R-free	0.27000
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	2.250
High resolution limit [Å]	2.170	2.170
Rmerge	0.105	0.210
Number of reflections	15058
<I/σ(I)>	15
Completeness [%]	76.6	14.4
Redundancy	14.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5 *	293	protein solution is mixed in a 3:2 ratio with well solution *
1	Vapor diffusion *	6.5 *	293	protein solution is mixed in a 3:2 ratio with well solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	succinate	10 (mM)
3	1	drop	beta-OG	1.5 (%)
4	1	drop		1 (mM)
5	1	reservoir	MPD	25-29 (%(v/v))
6	1	reservoir		1 (mM)
7	1	reservoir	bis-Tris	0.1 (M)