1QCW

Flavocytochrome B2, ARG289LYS mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-03-31
Detector	RIGAKU RAXIS
Spacegroup name	P 32 2 1
Unit cell lengths	162.569, 162.569, 112.011
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	500.000 * - 2.750
Rwork	0.204
R-free	0.22100 *
RMSD bond length	0.012 *
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.870
High resolution limit [Å]	2.750	2.750
Rmerge	0.078	0.416
Number of reflections	36062 *
<I/σ(I)>	7.8
Completeness [%]	80.8	75.2
Redundancy	1.7	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5 *	288	pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 288K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	MES	20 (mM)
4	1	reservoir	sodium sulfite	50 (mM)
5	1	reservoir	PEG4000	12 (%)