1QCQ

UBIQUITIN CONJUGATING ENZYME

Replaces:  2UCE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1992-12-16
Detector	NICOLET
Spacegroup name	P 41 21 2
Unit cell lengths	66.900, 66.900, 91.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.700
Rwork	0.240
R-free	0.29600
RMSD bond length	0.008
RMSD bond angle	23.400 *
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	X-PLOR
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.870
High resolution limit [Å]	2.700	2.700
Rmerge	0.134	0.368
Total number of observations	22434 *
Number of reflections	5940
<I/σ(I)>	10.2
Completeness [%]	97.0	87
Redundancy	3.8	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.8		PEG 8000, MAGNESIUM ACETATE, CACODYLATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP
1	Vapor diffusion *	5.8		PEG 8000, MAGNESIUM ACETATE, CACODYLATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP
1	Vapor diffusion *	5.8		PEG 8000, MAGNESIUM ACETATE, CACODYLATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP
1	Vapor diffusion *	5.8		PEG 8000, MAGNESIUM ACETATE, CACODYLATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	10 (%)
3	1	reservoir	magnesium acetate	0.2 (M)
4	1	reservoir	cacodylate	0.1 (M)