1QCG

LOW TEMPERATURE STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-06-08
Detector	RIGAKU RAXIS
Spacegroup name	P 1
Unit cell lengths	48.680, 49.090, 64.560
Unit cell angles	67.95, 82.90, 65.37

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.208
Rwork	0.191
R-free	0.27000
RMSD bond length	0.007
RMSD bond angle	22.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.170
High resolution limit [Å]	2.000	2.100
Rmerge	0.059	0.137
Number of reflections	25956
Completeness [%]	88.5	78
Redundancy		1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	16-18% PEG 4000, 0.1M CACL2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-20 (mg/ml)
2	1	reservoir	PEG4000	16-18 (%)
3	1	reservoir		0.1 (M)
4	1	reservoir	Tris-HCl	50 (mM)