1QC9
THE CRYSTALLOGRAPHIC STRUCTURE OF RESTRICTION ENDONUCLEASE ECO RI AT 3.3 A IN THE ABSENSE OF DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS-NICOLET X100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.680, 127.350, 49.870 |
| Unit cell angles | 90.00, 98.57, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| Rwork | 0.251 |
| R-free | 0.29500 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.573 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | MERLOT |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 3.060 |
| Number of reflections | 17576 |
| <I/σ(I)> | 20.41 |
| Completeness [%] | 72.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
