1QC7
T. MARITIMA FLIG C-TERMINAL DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-04-01 |
| Detector | SDMS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.680, 62.440, 88.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.200 |
| Rwork | 0.235 |
| R-free | 0.30400 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.653 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.101 | 0.413 |
| Total number of observations | 56990 * | |
| Number of reflections | 14359 | |
| <I/σ(I)> | 6 | |
| Completeness [%] | 97.1 * | 92.4 * |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.2 | 13 * | ISOPROPANOL, pH 5.2, temperature 286K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | isopropanol | 16 (%) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 10 (mM) |
