1QC7

T. MARITIMA FLIG C-TERMINAL DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1998-04-01
Detector	SDMS
Spacegroup name	P 21 21 21
Unit cell lengths	50.680, 62.440, 88.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.200
Rwork	0.235
R-free	0.30400
RMSD bond length	0.016
RMSD bond angle	1.653
Data reduction software	SDMS
Data scaling software	SDMS
Refinement software	XTALVIEW

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.101	0.413
Total number of observations	56990 *
Number of reflections	14359
<I/σ(I)>	6
Completeness [%]	97.1 *	92.4 *
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.2	13 *	ISOPROPANOL, pH 5.2, temperature 286K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	isopropanol	16 (%)
3	1	reservoir	sodium acetate	100 (mM)
4	1	reservoir		200 (mM)
5	1	reservoir	EDTA	1 (mM)
6	1	reservoir	dithiothreitol	10 (mM)